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In addition, the threshold degree of CYC9 or CRK12 essential for each of such functions may very well be distinctive, in order that depletion of CYC9 or CRK12 can be enough to disrupt cytokinesis or endocytosis, but not both equally.To analyze the subcellular localization from the CRK12 protein, a pEarleyGate104 vector was employed for a transient